Physicochemical and biopharmaceutical characterization of new sulfonamide derivatives of gallic acid

Gallic acid (GA) is known for its antioxidant activitywhichis restricted due to low oral permeability. In this project the carboxylic group of was substitutedwith sulfonamide and hydroxyl groups were methylated which resulted insignificantly (p<0.01) increased permeability 3,4,5-trimethoxybenzenesulfonamide (TMBS) 3,4,5-trihydroxybenzenesulfonamide (THBS) over GA, inParallel Artificial Membrane Permeability Assay studies with simulatedgastrointestinal fluids Human intestinal epithelial cells HIEC-6 cells.Biochemical confirmed TMBS O-demethylated by CYP2D6. THBS GAhad activity concentration in DPPH assaywhile indicated lower at all tested concentration. Theantioxidant greater than GA mainlyrelated O-demethylation CYP2D6.